General information

The muscle pathology laboratory is part of the Department of Pathology. It is involved in diagnostics and research concerning neuromuscular disorders.

Laboratory tests

Routinely enzymehistochemical and morphometric examination is performed on frozen muscle samples. The following stains are routinely performed: HE, Gomori trichrome , Sudan Black, PAS, acid phosphatase, ATPase at pH 4.2, 4.6 and 10.3, NADH, SDH and COX,. Based on the initial findings the examination is completed with immunohistochemistry and electron microscopy.

Shipping and Handling

The material for enzyme and immunohistochemistry must be snap frozen in isopentane -140 C chilled in liquid nitrogen. When frozen this way it can be sent on dry ice.
For electron microscopy fixation in 2 % buffered glutaraldehyde is necessary.

Reporting of results

A preliminary report is available at the day following the biopsy. A final report is sent out as soon as all the test results have been obtained. This is routinely within 3 weeks from the date of receipt of the request form with material.


In accordance with Dutch regulations the costs are 89 Euro for histopathological examination, 357 Euro for enzymehistochemical examination, 176 Euro for morphometric examination, 357 Euro for immunohistochemical examination and 375 Euro for electron microscopy.